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MBios 454

LAB

TOPIC

1

Large Scale Alkaline Lysis Plasmid Preparation

2

Large Scale Alkaline Lysis Plasmid Preparation

3

Large Scale Alkaline Lysis Plasmid Preparation

4

Determination of Plasmid DNA Concentration Using Spectrophotometry and Agarose Gel Electrophoresis

5

Determination of Plasmid DNA Concentration Using Spectrophotometry and Agarose Gel Electrophoresis

6

Cloning Design for pMB454 (bioinformatics exercise)

7

Restriction Enzyme Analysis and Mapping of pPC104

8

Restriction Enzyme Analysis and Mapping of pPC104

9

Preparation of Vector pUC8 for Cloning

10

Purification of fepA by Isolation of a DNA Restriction Fragment

11

Purification of fepA by Isolation of a DNA Restriction Fragment

12

Making Cells Chemically Competent for Transformation

13

Cloning pMB454

14

Cloning pMB454

15

Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method

16

Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method

17

Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method

18

Southern Blotting

19

Southern Blotting

20

Southern Blotting

21

Southern Blotting

22

Isolation of E. coli Genomic DNA

23

Isolation of E. coli Genomic DNA

24

Isolation of E. coli Genomic DNA

25

PCR Primer Design (bioinformatics exercise)

26

PCR Amplification of lacZ

27

DNA Fingerprinting

28

DNA Fingerprinting

 


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