LAB |
TOPIC |
1 |
Large Scale Alkaline Lysis Plasmid Preparation |
2 |
Large Scale Alkaline Lysis Plasmid Preparation |
3 |
Large Scale Alkaline Lysis Plasmid Preparation |
4 |
Determination of Plasmid DNA Concentration Using Spectrophotometry and Agarose Gel Electrophoresis |
5 |
Determination of Plasmid DNA Concentration Using Spectrophotometry and Agarose Gel Electrophoresis |
6 |
Cloning Design for pMB454 (bioinformatics exercise) |
7 |
Restriction Enzyme Analysis and Mapping of pPC104 |
8 |
Restriction Enzyme Analysis and Mapping of pPC104 |
9 |
Preparation of Vector pUC8 for Cloning |
10 |
Purification of fepA by Isolation of a DNA Restriction Fragment |
11 |
Purification of fepA by Isolation of a DNA Restriction Fragment |
12 |
Making Cells Chemically Competent for Transformation |
13 |
Cloning pMB454 |
14 |
Cloning pMB454 |
15 |
Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method |
16 |
Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method |
17 |
Rapid Isolation of Plasmid DNA by the Boiling Miniprep Method |
18 |
Southern Blotting |
19 |
Southern Blotting |
20 |
Southern Blotting |
21 |
Southern Blotting |
22 |
Isolation of E. coli Genomic DNA |
23 |
Isolation of E. coli Genomic DNA |
24 |
Isolation of E. coli Genomic DNA |
25 |
PCR Primer Design (bioinformatics exercise) |
26 |
PCR Amplification of lacZ |
27 |
DNA Fingerprinting |
28 |
DNA Fingerprinting |