LAB |
TOPIC |
1 |
Lab orientation and expectations; buffers and dilutions |
2 |
Lab safety orientation; make buffers; check pipettor accuracy |
3 |
Isolate bacteriophage from sewage-enrichment |
4 |
Phage isolation-centrifugation/filtration, plaquing |
5 |
Count plaques, discuss one step growth curve |
6 |
Perform one step growth curve |
7 |
Count plaques, calculate data |
8 |
Lysogeny, lambda phage, pick λ |
9 |
Verification of lysogeny |
10 |
Grow and induce lysogenic λ, isolate to CHCL 3 |
11 |
DNA extraction, PEG ppt, RR1 plaque assay |
12 |
Centrifuge, extraction; ppt, RE digest |
13 |
Run gels, intro to cell culture and cell types |
14 |
Introduction to Sf9 culture and baculovirology |
15 |
Freeze cells, infect cells with baculovirus |
16 |
Harvest & titrate virus, mammalian cell culture |
17 |
Cell counting |
18 |
Pass cells & infect BT cells with IBRV |
19 |
Freeze BT cells, Reed Muench 50% end point |
20 |
Sensitivity to organic solvents |
21 |
Sensitivity to low pH |
22 |
Read results, calculate end points |
23 |
Electron microscopy of viruses |
24 |
Titrate NDV in fertilized eggs |
25 |
Candle eggs, calculate EID 50 |
26 |
Review, lab clean up |
27 |
Practical final |
28 |
Written final; data books collected |
