LAB |
TOPIC |
1 |
Lab orientation and expectations; buffers and dilutions |
2 |
Lab safety orientation; make buffers; check pipettors accuracy |
3 |
Immune cells and organs; blood, make blood smears; differential blood counts |
4 |
Examine pathology slides; writing in the major |
5 |
Antigen-Antibody reactions, agglutination, blood typing, immunodiffusion reactions |
6 |
Immunocytochemical staining of B cells, day 1 |
7 |
Immunocytochemical staining of B cells, day 2, examine slides |
8 |
Discuss results |
9 |
Jerne plaque assay |
10 |
Coat plates for ELISA, rabbit handling and bleeding video, confocal microscopy |
11 |
Perform ELISA |
12 |
ELISA results, grocery store ELISA |
13 |
Affinity chromatography; prepare recombinant antigen |
14 |
Affinity purify antigen |
15 |
Spectrophotometrically quantitate protein yield |
16 |
SDS-PAGE; western blotting; pour practice gels |
17 |
Run gels for Coomassie blue staining |
18 |
Destain gels and calculate molecular weights |
19 |
Run gels for western blot; transfer to PVDF |
20 |
Probe membranes with antibody, develop with ECL |
21 |
Discuss Results, discuss flow cytometry |
22 |
Stain cells for flow cytometry |
23 |
Examine cells by flow cytometry |
24 |
Discuss flow results |
25 |
Forensics, lab clean up |
26 |
Case studies, Review |
27 |
Practical final |
28 |
Written final; data books collected |
