LAB |
TOPIC |
1 |
Introduction: Handling Pipettors |
2 |
DNA Isolation, Genomic Spectrophotometric Analysis |
3 |
DNA Isolation, Plasmid Both Methods |
4 |
DNA Isolation, Plant Spectrophotometric Analysis |
5 |
Restriction Mapping Plan and Set up Restriction Digests |
6 |
Gel Electrophoresis |
7 |
DNA Analysis and Electrophoresis Sign up for Competent Cell Time |
8 |
Make Competent Cells Sign up for Plate Number and Antibiotic |
9 |
Cell Transformation |
10 |
Count Colonies and Calculate Transformation Efficiency |
11 |
Human Chromosomes |
12 |
Pictures of Stained Chromosomes, Making Spreads for Chromosome Painting
(Optional Experiment) |
13 |
Chromosome Painting-Hybridization
(Optional Experiment) |
14 |
Examine Chromosome Painting Slides. Inheritance of Mutant Traits in Drosophila
|
15 |
Plant Relationships: Rubisco, PCR, Finish Human Chromosomes |
16 |
Electrophoresis of Rubisco PCR Products and Plan Digests of All Eight Plants. Finish Human Chromosomes, Remove Parents From Drosophila Crosses |
17 |
Rubisco, Restriction Digests and Electrophoresis |
18 |
Score Drosophila F 1. Set up F 1 Cross |
19 |
Remove Parents From F 1 Cross |
20 |
Finish Drosophila Experiment. Count F 2 |
21 |
Finish Drosophila Experiment. Count F 2. Get Class Data |
22 |
Plan Mutants in the Lac Operon of E. Coli |
23 |
Pour Plates (Optional Experiment) |
24 |
Mutants and Survival Curve |
25 |
Plate Mutants. Count Colonies |
