LAB |
TOPIC |
1 |
Basic Methods: Use of pipettman, balances, spectrophotometers, pH meter |
2 |
Colorimetric Assay for Glucose, Excel Graphing |
3 |
Protein Concentration Determination: BCA assay, Bradford and A280 |
4 |
Ion Exchange Chromatography I: Make buffers, pour DEAE-cellulose column, Prepare gradient maker |
5 |
Ion Exchange Chromatography II: Run DEAE-cellulose column |
6 |
SDS-PAGE on DEAE-cellulose Column Fractions |
7 |
Lactate Dehydrogenase Purification I: Column and buffer preparation |
8 |
Lactate Dehydrogenase Purification II: Extraction from chicken breast, ammonium sulfate precipitation, LDH activity assay |
9 |
Lactate Dehydrogenase Purification III: Cibacron Blue affinity column |
10 |
Lactate Dehydrogenase Purification IV: SDS-PAGE |
11 |
Lactate Dehydrogenase Purification V: Purification table |
12 |
Enzyme Kinetics I: Tyrosinase Michaelis Menton kinetics |
13 |
Enzyme Kinetics II: Tyrosinase, inhibitor studies |
14 |
Enzyme Kinetics III: effect of pH on LDH activity |
15 |
Continue Enzyme Kinetics III: Effect of pH on LDH activity |
16 |
Lysozyme Crystallization |
17 |
Tour of NMR, MALDI, X-ray Crystallography Facilities |
18 |
MALDI Analysis of Peptide Fragments |
19 |
Chemical Modification of Proteins: Cysteine modification by DTNB |
20 |
Activity of Immobilized Peroxidase |
21 |
Protein Denaturation I: Sample preparation |
22 |
Protein Denaturation II: Chemical denaturation |
23 |
Protein Denaturation III: Thermal denaturation |
24 |
Western Blot I |
25 |
Western Blot II |
